The invention provides a chromatographic preparation method of a compound. The chromatographic preparation method of the compound comprises the steps of preparing seabuckthorn into a seabuckthorn extract, mixing the extract with silica gel, then loading the mixture into a column, and washing the column by taking petroleum ether as well as mixed liquid of petroleum ether and ethyl acetate which aremixed at different volume ratios as a mobile phase; and sequentially carrying out liquid chromatography analysis, normal phase chromatography preparation, liquid chromatography analysis and reverse phase chromatography preparation on the fraction obtained in the step (1), and finally carrying out liquid chromatography analysis on purity. The compound is extracted and prepared from the seabuckthorn extract for the first time, has an alpha-glucosidase inhibition effect, and can be applied to preparation of related products for reducing blood sugar.