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发明公开
CN110408707A 克隆自InDel片段的与猪毛色性状相关的分子标记 有权 转让

克隆自InDel片段的与猪毛色性状相关的分子标记
摘要：
本发明属于猪分子标记筛选技术领域，具体涉及克隆自InDel片段的与猪毛色性状相关的分子标记。该分子标记通过整合NCBI数据库和欧洲生物信息研究所中公开的包含58个品种的469头猪的重测序数据，对比猪参考基因组序列获得基因分型数据，根据全基因组关联分析(GWAS)得到一种与猪毛色性状相关的分子标记，即发生在Chr 11:50076946bp处的缺失，由原片段CTGGGAACTT缺失为C，具体核苷酸序列如SEQ ID NO:1所示。当个体基因型为CC时，有利于个体拥有渐变带毛色。本发明公开了筛选猪毛色性状相关分子标记的方法及其关联分析的应用，为猪毛色性状的标记辅助选择提供了新的InDel分子标记。
摘要（英）：

The invention belongs to the technical field of pig molecular marker screening, and in particular, relates to a molecular marker related to a pig hair color trait and cloned from an InDel fragment. According to the molecular marker, by integrating an NCBI database and re-sequencing data of 469 pigs including 58 varieties and published by the European Bioinformatics Institute, genotyping data are obtained by comparing reference genome sequences of pigs, and the molecular marker related to the pig hair color trait is obtained according to the whole genome association analysis (GWAS), that is, deletion occurs at Chr 11:50076946 bp, an original fragment CTGGGAACTT is deleted to C, and the specific nucleotide sequence is shown in SEQ ID NO:1. When an individual genotype is CC, the genotype is beneficial for the individual to have gradual hairy color. The invention discloses a method for screening the molecular marker related to the pig hair color trait and an application of correlation analysis, and provides the new InDel molecular marker for marker assisted selection of the pig hair color trait.

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