The invention discloses a pharmacokinetics and elimination detection method of sodium ceftiofur in chicken body. The method comprises the following steps: for the pharmacokinetics characteristics of four sodium ceftiofur products for injection in the yellow chicken body in one-day old, selecting a health chick in the one-day old for each medicine, subcutaneously injecting 0.1mg of sodium ceftiofurinto each chick, detecting desfuroyl ceffiofur (DFC) concentration in plasma through a high-performance liquid chromatography, researching the pharmacokinetics of the DFC in the yellow chicken body in one-day old, thereby realizing the research of the pharmacokinetics of the sodium ceftiofur in the yellow chicken body in one-day old.