The invention belongs to the field of tobacco planting, and particularly relates to a tobacco seedling stock preparation apparatus, comprising a rack, a cutting mechanism, a stock clamp, a clamp feeding mechanism and a tobacco seedling delivery mechanism. Industrialized work is realized with the tobacco seedling stock preparation apparatus; particularly, since strength support in cutting and limitin cutting direction and position are provided to tobacco seedlings through the clamp, the tobacco seedling stocks have the same cuts and are protected effectively; meanwhile, with cooperation between different drive mechanisms, cutting work of tobacco seedlings in batch can be realized well; further, with mechanical control of cutting tools, fixed overall operating environment and introduction of sterilize management, bacterial infection rate caused by cutting of the tobacco seedlings is decreased.