The invention relates to a method for quick breeding of mesembryanthemum crystallium tissue and belongs to the technical field of mesembryanthemum crystallium cultivation. The method includes: sterileseedling culture, callus culture, adventitious bud differentiation, transgenerational propagation, rooting and seedling strengthening, and transplanting. Mesophyll tissue of sterile seedlings is utilized, a blade is utilized for cutting, and exposed palisade tissue and spongy tissue are made to contact with a culture medium as much as possible. The sterile seedlings are cultivated for propagation, so that contamination rate and browning rate of callus are lowered, and experimental survival rate is increased; by the method, a lot of mesembryanthemum crystallium seedlings can be obtained through tissue propagation, so that industrialized seedling culture of mesembryanthemum crystallium is promoted.