The invention discloses an antibacterial/antifogging coating based on N-hydroxyethyl acrylamide and a preparation method thereof. Firstly, a random copolymer of the N-hydroxyethyl acrylamide, a quaternary ammonium salt and perfluoroalkyl methacrylate is synthesized through free radical polymerization, and then the random copolymer is thermo-crosslinked with a hexamethylene diisocyanate trimer under catalysis of dibutyltin dilaurate to form a film. The N-hydroxyethyl acrylamide with high hydratability and the positively-charged quaternary ammonium salt enable the coating to have the propertiesof bacterial adhesion resistance and sterilization, synergistic bacterial resistance is achieved, and the N-hydroxyethyl acrylamide with the high hydratability and the positively-charged quaternary ammonium salt both contain hydrophile groups so that the coating can be made to have the good antifogging property; the stability of the coating is improved advantageously through introduction of the perfluoroalkyl methacrylate; and the prepared coating is good in water resistance and high in transparency, can be used for the surfaces of various base materials, shows the good antibacterial propertyand antifogging property, and thus has practical application value in the field of medical devices.