The present invention discloses a method for preparing brush wires for an antibacterial and environmental protection industrial brush. The method comprises: placing glass fiber, epoxy resin, polyurethane resin and PBT resin in a dryer for drying, then performing slicing processing, and thoroughly mixing and stirring the dried mixed slice with the auxiliary agent and the antibacterial agent to perform melt extrusion processing; performing extrusion and shaping on the melt-plasticized material through a mouth model with an inner cavity as a plurality of grooves, and performing pulling and wiredrawing on the material to obtain a primary brush wire with a three-dimensional side as a plurality of ribs; performing twisting on the primary brush wire; and soaking the primary brush wire in a TPM9007 solution. The brush wire obtained by using the method provided by the present invention has excellent wear resistance and high elasticity, and also has excellent antibacterial and environmental protection performance; the brush wire surface has both a three-dimensional concave and convex structure and a thread structure, so that better cleaning can be performed, and the dirt which is peeled offduring brushing can be received; the brush wires obtained by using the method can be widely used in cleaning brushes and polishing brushes; and by using the thread structure and the concave and convexstructure, the to-be-extracted medium can be better extracted and received, and the application scope of the thread brush wires is effectively improved.