The invention discloses a preparation method of CuO-doped Bi2SiO5 polycrystal glass. According to the mole fraction, diboron trioxide, silicon dioxide, bismuth trioxide, manganese dioxide, copper oxide, tungsten trioxide, antimonous oxide and germanium dioxide are collected and mixed, and a mixture is obtained; the mixture is placed in a crucible at the preheating temperature of 800-900 DEG C, thetemperature is increased to 950-1,100 DEG C for temperature preservation, and glass liquid is obtained; the glass liquid is placed in a mold and formed, the mold is placed in an annealing furnace fortemperature preservation at 400-600 DEG C and cooled to the indoor temperature through furnace cooling, and the CuO-doped Bi2SiO5 polycrystal glass is obtained. According to the preparation method, the method conversion rate of the preparation method is high, the cost is low, no pollution is caused, and the human body cannot be damaged.