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发明授权
CN110455896B 金属有机骨架复合物比率电化学miR3123适体传感器的制备方法 失效 - 权利终止

金属有机骨架复合物比率电化学miR3123适体传感器的制备方法
摘要：
本发明公开了基于黑磷纳米片BPNSs和硫堇TH掺杂的铜基金属有机骨架Cu‑MOF复合物的比率电化学miR3123适体传感器的制备方法。将TH与Cu‑MOF前驱体反应制备TH/Cu‑MOF，滴涂BPNSs制备BPNSs/TH/Cu‑MOF，将其滴涂在电极上。二茂铁Fc标记的DNA单链适体吸附在BPNSs上，制得适体‑BPNSs/TH/Cu‑MOF。靶分子miR3123与DNA单链适体Fc‑DNA结合，使Fc‑DNA脱离BPNSs，引起Fc电化学信号减弱，而对TH信号影响甚微。拟合电流峰强度比率IFc/ITH与miR3123浓度之间的线性关系，构建比率电化学miR3123适体传感器。
摘要（英）：

The invention discloses a preparation method of a ratio electrochemical miR3123 aptamer sensor based on a Cu-MOF (copper-based metal organic framework) compound doped with BPNSs (black phosphorusnanosheets) and TH (thionine). The method comprises steps as follows: TH and a Cu-MOF precursor are subjected to a reaction for preparation of TH/Cu-MOF, and BPNSs/TH/Cu-MOF is prepared by drip coating ofthe BPNSs and applied to an electrode by drip coating; DNA single-stranded aptamers marked with Fc (ferrocene) are adsorbed on the BPNSs, and aptamer-BPNSs/TH/Cu-MOF is prepared; a target moleculemiR3123is bonded with the DNA single-stranded aptamers Fc-DNA, Fc-DNA is enabled to be separated from the BPNSs, and therefore, Fc electrochemical signals are weakened while TH signals are slightly affected; the ratio electrochemical miR3123aptamer sensor is constructed by fitting linear relation of current peak intensity ratio IFc/ITH and miR3123 concentration.

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