The invention discloses preparation and application of a nitrogen-containing heterocyclic mixed-source terpenoids with remarkable anti-tumor activity. The substance is separated from a fungus, Cliocybe clavipes, and is characterized in that the seven-membered nitrogen heterocyclic is fused with benzo ten-membered carbon ring. The substance has cytotoxic effect, apoptosis induction effect and cellcycle influence effect on hematoma and solid tumor, and application of the seven-membered nitrogen heterocyclic fused benzo ten-membered carbon ring in clinical prevention and treatment of tumors.