The invention provides a test method for testing the anti-loosening performance of a corrugated plate connecting piece. The method comprises the following steps of: selecting two corrugated plates, and enabling the two corrugated plates to be in fastening connection through a corrugated plate connecting piece, so as to manufacture a corrugated plate connecting piece anti-loosening test piece; installing the corrugated plate connecting piece anti-loosening test piece on a transverse vibration testing machine, and applying a pre-clamping force; starting the transverse vibration testing machine to load the corrugated plate connecting piece anti-loosening test piece, recording the magnitude of the clamping force and the vibration frequency of the corrugated plate connecting piece anti-loosening test piece, and closing the transverse vibration testing machine until the pre-clamping force reaches a preset value; selecting a plurality of groups of corrugated plate connecting piece anti-loosening test pieces with different models, and repeating the test steps mentioned above; and arranging test data, drawing a relation change curve graph of the vibration times and the clamping force for the corrugated plate connecting piece anti-loosening test pieces with different models, and performing comparative analysis to obtain the anti-loosening performance of the corrugated plate connecting piece anti-loosening test pieces.