The invention relates to a construction method of a biological regulating pond of a prawn circulating water breeding system, and belongs to the field of aquaculture sewage treatment. The method comprises the steps of construction of the biological regulating pond and implementation of a cultivation method of a biological membrane, wherein the biological regulating pond is divided into two stages,the first-stage biological regulating pond body and the second-stage biological regulating pond body are filled with an elastic hairbrush and a multi-space suspended microorganism adherance respectively, the lower portions of the first-stage biological regulating pond body and the second-stage biological regulating pond body are communicated with each other, and the bottoms of the first-stage biological regulating pond body and the second-stage biological regulating pond body are of a conical structure and are provided with central bottom sewage outlets; water in the later period of prawn circulating water breeding is utilized for the culture of the biological membrane after ammonium chloride is added, and after about 28 days of culture, the biological membrane is formed; the conical bottom structure of the biological regulating pond facilitates sewage discharge, cleaning and maintenance; the construction method has the advantages that the operation is simple, the addition of excessivecomponents and complicated operation are not needed, the culture of the biological membrane is quick and efficient, maintenance is convenient, the cost is low, and long-time and efficient running canbe conducted.