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发明公开
CN110499359A 一种快速鉴别日本血吸虫，曼式血吸虫，东毕吸虫的LF-RPA方法及其应用 审中-实审

一种快速鉴别日本血吸虫，曼式血吸虫，东毕吸虫的LF-RPA方法及其应用
摘要：
本发明公开了一种快速鉴别日本血吸虫，曼式血吸虫，东毕吸虫的LF-RPA方法及其应用；还公开了快速检测试剂盒及检测方法。本发明的有益效果为：本发明提供的用于检测日本血吸虫，曼式血吸虫，东毕吸虫的引物对和探针及快速检测试剂盒，是建立在分子生物学基础上的，该引物和探针能够特异性的扩增日本血吸虫，曼式血吸虫，东毕吸虫，提供的快速检测日本血吸虫，曼式血吸虫，东毕吸虫核酸的试剂盒，敏感性高于PCR，特异性强，检测速度快，结果的判定操作极其简便；本发明提供检测方法和检测试剂盒，结合已有的核酸快速提取方法，能够达到快速鉴定、检测日本血吸虫，曼式血吸虫，东毕吸虫的目的。
摘要（英）：

The invention discloses a LF-RPA method for quickly identifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis and application thereof and further discloses a quick detection kit and a detection method. A primer pair, probe and quick detection kit for detecting the schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis are established on the basis of molecular biology. The primer and probe can be used for specifically amplifying schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis, the kit for quickly detecting nucleicacids of schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis has the sensitivity higher than that of PCR, has strong specificity and high detection speed, and is extremely simple and convenient in result determination operation. The detection method and detection kit combined with an existing nucleic acid quick extraction method can achieve the aim of quickly identifyingand detecting schistosoma japonicum katsurada, schistosoma mansoni and orientobilharziasis.

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