The invention relates to the field of molecular biology, in particular to a gene for improving xylose utilization rate, a recombinant vector, a recombinant cell and an application method thereof. Theinvention discloses the gene for improving xylose utilization rate. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1; the gene is amplified through an amplification technology; the recombinant vector containing the gene is constructed and converted into escherichia coli competent cells through a heat shock method to obtain escherichia coli engineering bacteria, the expression levelof xylose isomerase in escherichia coli is enhanced, the xylose metabolism capacity of escherichia coli is improved, the concentration of lysine in a product can be remarkably improved, and the saccharic acid conversion rate is increased.