The invention provides a pyridine bridged multi-response acceptor molecule as well as a synthesis method and an application thereof. The acceptor molecule is characterized in that rhodamine B spirolactam and ferrocene are linked through a pyridine bridged structure, one end of the pyridine bridged structure is linked with nitrogen of an amide group in rhodamine B spirolactam, the other end of thepyridine bridged structure is linked with carbon of ferrocene, and the structural formula of pyridine bridged groups is shown in the description. The acceptor molecule has the electrochemical, ultraviolet, fluorescent and colorimetric multi-response function, Hg<2+> ions in aqueous phase are selectively and rapidly detected with cyclic voltammetry, ultraviolet-visible and fluorescence spectroscopymethods, the lowest detection limit is 4.14*10<-7>M, and the acceptor molecule has broad application prospects.