The invention discloses an echinococcus shiquicus detection kit based on a RPA technology and application of the echinococcus shiquicus detection kit. A set of primer and probe combination capable ofbeing used for RPA detection is designed and screened according to the gene sequence of mitochondria of echinococcus shiquicus, and an echinococcus shiquicus specificity real-time fluorescent RPA detection method is established. According to the method, recombinant enzymes and single-stranded binding proteins are adopted to cooperate at the normal temperature to achieve specific binding of a primer and a template, and under the effect of DNA polymerases, the primer is extended to generate new DNA strands. The echinococcus shiquicus specificity fluorescent RPA detection method is easy, convenient and quick to operate, high in specificity, and suitable for site quick detection.