The invention discloses a preparation method and application of a cobalt and nitrogen doped three-dimensional ordered porous carbon catalyst. According to the method, PS balls with different particlesizes are firstly synthesized and the PS balls are manufactured into a template. The template is impregnated in a methanol solution with cobalt nitrate hexahydrate and 2-methylimidazole. ZIF-67 is in-situ-grown on the template. The template is washed with tetrahydrofuran and centrifuged to obtain an ordered porous ZIF-67 precursor, and cobalt and nitrogen doped three-dimensional ordered porous carbon catalyst is obtained after carbonization treatment. The prepared catalyst is a three-dimensional carbon material with regular structure and clear particles and has the advantages of ordered pores,high specific surface area, high activity, high stability, etc. The method provides great possibility for synthesizing oxygen reduction electrocatalytic materials with controllable pores, high specific surface area and good durability. The used raw materials are easy to obtain and low in price so as to have industrial application prospect.