The invention discloses a method and a device for calculating regional stratum lifting amplitude. The method comprises: recovering the paleo-burial depth of a regional stratum before and after tectonic movement, and obtaining a two-stage paleo-burial depth plane contour line distribution diagram; carrying out plane gridding processing on the two-stage ancient burial depth data to obtain the ancient burial depth of each grid point; calculating the absolute lifting amplitude of the stratum before and after tectonic movement according to the paleo-burial depth of each grid point; according to theabsolute lifting amplitude and geological time experienced by tectonic movement, obtaining the stratum lifting rate; and selecting the maximum absolute lifting amplitude generated by the tectonic movement to obtain the relative lifting amplitude of the stratum before and after the tectonic movement. According to the method, the advantages of the quantitative evaluation method are fully utilized to analyze the lifting and sedimentation amplitude of the regional stratum, and the breadth and accuracy of stratum tectonic movement evaluation are improved to a great extent.