The invention belongs to the technical field of soilless cultivation, and discloses an organic vegetable cultivation method and a cultivation groove adopted by the same and application. A commodity organic substrate and other substrates are used for matching to form a high-quality organic substrate, and materials are coco fiber, bark and the like; high-quality, high-yield and other vegetable varieties are selected; seeds are soaked with hot water and then placed into an incubator for germination acceleration, and after germination, the seeds are sown into plugs for growing seedlings; after seedlings grow to three leaves and one heart, and after the root system compacts the substrate lump, the seedlings are planted into a built cultivation groove in time; the cultivation groove is designedspecially and divided into an upper layer and a lower layer, the upper layer is used for planting vegetable crops, and the lower layer is adopted as a water holding groove; before planting, the organic substrate landed into the cultivation groove is watered thoroughly; after vegetable seedlings are planted and survive, clean flowing water is injected into the lower layer of the cultivation groove,and the water level is observed and supplemented regularly; after planting, scientific and reasonable temperature, illumination, humidity and water management can be performed inside a greenhouse. Organic vegetable crops with the high economic value can be produced.