The invention discloses a eustoma grandiflorum seedling raising method capable of eliminating rosette characters through regulation and control of temperature and light. The method solves the problemsof eustoma grandiflorum seed dormancy and obstruction on seedling rosettes, field planting and flowering. The method comprises the steps of low-temperature moisturizing pre-culture after sowing, regulation and control of light and temperature during germination acceleration culture and strong seedling culture, low-temperature vernalization treatment on small seedlings before harvesting and illumination regulation and control. According to the method, the average germination rate of seeds reaches 96.4%, the average rosette rate at the seedling stage is 0.8%, and the average rosette rate at theflowering and planting stage is 0.91%. Compared with the prior art, the eustoma grandiflorum seedling raising method has the advantages that the average germination rate of the seeds is increased by9.58%, the average rosette rate at the seedling stage is reduced by 78.1%, and the average rosette rate at the flowering and planting stage is reduced by 88.3%.