The invention relates to a perfusion and liquor change system for an isolated organ. The system comprises a pipeline power circulation subsystem, a liquor change subsystem and a control subsystem, wherein the pipeline power circulation subsystem is in through connection with an isolated organ perfusion system; the liquor change subsystem is in through connection with the pipeline power circulationsubsystem; the control subsystem is independently in communication connection with the pipeline power circulation subsystem and the liquor change subsystem; the liquor change subsystem is used for obtaining and filtering the perfusion liquor of the isolated organ perfusion system and is also used for transmitting replenishing liquor and the filtered perfusion liquor to the isolated organ perfusion system through the pipeline power circulation subsystem; and the control subsystem is used for obtaining waste liquor generation data in preset time, obtaining the replenishing data of the replenishing liquor according to the waste liquor generation data, and driving the liquor change subsystem to transmit the replenishing liquor to the pipeline power circulation subsystem according to the replenishing data. By use of the system, the perfusion and liquor change balance of the system can be realized, an operation process of the liquor change of the perfusion liquor is simplified, a liquor change error is reduced, liquor change accuracy is improved, and meanwhile, secondary pollution in a liquor change process can be avoided.