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发明公开
CN110564712A 一种用于丝状真菌液体发酵产α-半乳糖苷酶的培养基及其应用 审中-实审

一种用于丝状真菌液体发酵产α-半乳糖苷酶的培养基及其应用
摘要：
本发明涉及用于丝状真菌液体发酵产α-半乳糖苷酶的培养基及其应用，可有效解决丝状真菌液体发酵产α-半乳糖苷酶的培养基及其用于制备α-半乳糖苷酶的问题，该培养基由：乳糖12.0～18.0 g、牛肉膏8.0～12.0 g、MgSO4·7H2O 0.8～1.2g、Na2HPO4 0.2～0.8g、MnCl2 0.8～1.2g和水苏糖0.6～1.0g制成，将各组分用蒸馏水溶解，混匀，用盐酸或氢氧化钠调节pH为7.1～7.5，加蒸馏水至1000mL，121℃灭菌25分钟，冷却即成；本发明原料丰富，制备方法简单，显著提高α-半乳糖苷酶酶活性，α-半乳糖苷酶酶活力有显著提高，液体发酵发酵周期短、控制培养条件容易、易于实现自动化、不受季节限制等显著优点，为工业化生产奠定基础，经济和社会效益巨大。
摘要（英）：

The invention relates to a culture medium for producing alpha-galactosidase through filamentous fungus liquid fermentation and an application thereof, which can effectively solve the problem of the culture medium for producing alpha-galactosidase through filamentous fungus liquid fermentation and the application of the culture medium for preparing alpha-galactosidase. The culture medium is composed of: 12.0 to 18.0 g of lactose, 8.0 to 12.0 g of a beef extract, 0.8 to 1.2 g of MgSO4.7H2O, 0.2 to 0.8 g of Na2HPO4, 0.8 to 1.2 g of MnCl2 and 0.6 to 1.0 g of stachyose. The preparation method comprises the following steps: dissolving the components with distilled water, uniformly mixing the materials, regulating the pH value to 7.1-7.5 with hydrochloric acid or sodium hydroxide, adding distilled water to 1000mL, sterilizing the material at 121 DEG C for 25 minutes, and cooling the product. The method has the remarkable advantages that the raw materials are rich, the preparation method is simple, the enzyme activity of the alpha-galactosidase is remarkably improved, the enzyme activity of the alpha-galactosidase is remarkably improved, a liquid fermentation period is short, the culture conditions are easy to control, automation is easy to achieve, season limitation is avoided, a foundation is laid for industrial production, and economic and social benefits are huge.

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