The invention relates to temperature adjustment equipment for mechanical perfusion of an in-vitro lung, a method and a device. The temperature adjustment equipment for mechanical perfusion of the in-vitro lung comprises a liquid storage vessel, a membrane lung liquid circulating device, a temperature sensor, temperature adjusting equipment and main control equipment. The main control equipment isused for acquiring temperature data acquired by the temperature sensor and carrying out digital filtering processing on the temperature data, so as to obtain filtered temperature data; and the filtered temperature data and a target temperature are subjected to PID (Proportion-Integration-Differentiation) adjusting processing so as to obtain a processed result, and the temperature of the temperature adjusting equipment is adjusted according to the processed result. According to the temperature adjustment equipment, the method and the device, perfusate can be subjected to real-time temperature adjustment during in-vitro lung perfusion, and thus, the accuracy of temperature monitoring is improved.