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发明公开
CN110592069A 一种石油烃降解菌固定化菌剂的制备方法及固定化菌剂 审中-实审

一种石油烃降解菌固定化菌剂的制备方法及固定化菌剂
摘要：
本发明属于生物技术领域，尤其涉及一种石油烃降解菌固定化菌剂的制备方法及固定化菌剂，具体制备方法包括以下步骤：其由Gordonia alkanivorans W33(菌种保藏号，CGMCC No.6845)菌悬液和作为载体的蛭石组成，Gordonia alkanivorans W33菌悬液与蛭石的体积/质量比为：每10-30ml的菌悬液中加入10g的蛭石粉；最终每克固定化菌剂含有(2.0-5.0)×106CFU的Gordonia alkanivorans W33菌体。本发明提供一种利用蛭石粉的优良的性能，以石油烃降解菌GordoniaalkanivoransW33为目标的石油烃降解菌固定化菌剂的制备方法及固定化菌剂。
摘要（英）：

The invention belongs to the technical field of biology, and particularly relates to a preparation method of a hydrocarbon degradation bacterium (gordonia alkanivorans) immobilized microbial agent andthe immobilized microbial agent. The concrete preparation method comprises the following steps that the immobilized microbial agent is prepared from a gordonia alkanivorans W33 (the strain preservation number is CGMCC No.6845) bacterial suspension and vermiculite as a carrier, wherein according to the volume/mass ratio of the gordonia alkanivorans W33 bacterial suspension to the vermiculite, 10gof vermiculite powder is added into per 10 to 30ml of the bacterial suspension; and finally, per 1g of the immobilized microbial agent comprises (2.0 to 5.0)*10<6>CFU of gordonia alkanivorans W33 thalli. The invention provides the preparation method of the hydrocarbon degradation bacterium (gordonia alkanivorans) immobilized microbial agent using excellent performance of the vermiculite powder andusing hydrocarbon degradation bacteria gordonia alkanivorans W33 as a target, and the immobilized microbial agent.

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