The invention discloses a method for determining the flow conductivity of a hydraulically-created prop fracture of a tight sand reservoir by an experiment. The method comprises the following steps that an exposed rock sample of the reservoir is acquired, engraved rock samples with the real morphology of the surface of the hydraulically-created fracture are manufactured in batches through a fracture surface reconstruction technique, the sanding concentrations of different positions of the prop fracture are determined according to field construction parameters, and the flow conductivity of the prop fracture is tested after a propping agent with the corresponding concentration is laid on the engraved rock samples. According to the method, the seepage form of the underground prop fracture formed after hydraulic fracturing of the tight sand reservoir is truly simulated, the flow conductivity and distribution condition of the prop fracture are accurately evaluated, and a basis is provided for optimizing fracturing construction parameters.