The invention provides a cleaning and disinfection device for live chicken baskets. The cleaning and disinfection device for the live chicken baskets comprises a transmission mechanism, wherein the transmission mechanism comprises a conveying belt; a plurality of rolling bars are arranged on the outer surface of the conveying belt; a roller is rotationally connected to a support; a stepper mechanism is arranged on the support; the stepper mechanism is located below the conveying belt; the stepper mechanism comprises a rack; tooth matched with the rolling bars of the conveying belt are arrangedon the top surface of the rack; at least two groups of drive structures are connected to the bottom surface of the rack; a disinfection device and a washing device are arranged above the transmissionmechanism; a water tank is communicated to the disinfection device and the washing device through a water outlet pipe separately; a circular disc is rotationally connected to the end parts of the water outlet pipes of the disinfection device and the washing device through rotary mechanisms; the interior of each circular disc is hollow and communicates with a water outlet pipe; a plurality of spray heads are arranged on the bottom surface of each circular disc; a sewage tank is arranged below the conveying belt; and a filter plate is arranged in the sewage tank. The cleaning and disinfection device for the live chicken baskets has the beneficial effects of being reasonable in design, simple in structure, safe and reliable, and capable of increasing the working efficiency, and increasing the bio-safety level of a livestock farm.