A film fabrication device of schizothorax embryo chromosome comprises a bearing platform, wherein a first square box is arranged at an upper part of the bearing platform, an upper end of a first plastic flexible pipe is connected with a first water outlet in a lower part of the first square box, and a first blind hole is horizontally formed in a position, at a right side of a middle part of the first through hole, of the bearing platform. When the device works, a cell suspension liquid is added through a liquid injection hole of the first square box, the liquid injection hole is enclosed, thecell suspension liquid flows into the first plastic flexible pipe, a first liquid transmission pipe and a first dropping pipe, the cell suspension liquid is extruded out of the first dropping pipe anddrops onto a glass slide, the first plastic flexible pipe is recovered to an original shape when a first press rod moves rightwards, the pressure in the first square box is reduced, air enters from afirst one-way air valve, the air can enter only through the first one-way air valve, and the first dropping pipe is enabled to be filled with the cell suspension liquid all the way. Liquid dropping and glass slide transmission can be simultaneously performed by rotating a rotation shaft, and multiple functions are achieved; and automatic control is achieved by a controller, and the film fabrication device is high in automation, relatively simple in structure and relatively low in cost.