The invention provides an intelligent mobile online sampling and sample preparing vehicle which comprises a vehicle body, a storage hopper, an automatic sampling and feeding device, a conveying mechanism, a sample material guide groove, a cutting and sampling device, a waste sample conveying mechanism and a controller; the vehicle further comprises a sample collecting and conveying device electrically connected with the controller; and the sample collecting and conveying device comprises a first sample conveying mechanism arranged at one side of the waste sample conveying mechanism, a second sample conveying mechanism inclinedly arranged in the tail end of the conveying direction of the first sample conveying mechanism, a lifting mechanism arranged at the tail end of the conveying direction of the second sample conveying mechanism, and an auxiliary sample collector connected with the position opposite to the conveying direction of the first sample conveying mechanism. The intelligent mobile online sampling and sample preparing vehicle can realize automatic sampling and sample preparing, the prepared samples are uniform, errors are low, the sampling quality is high, and the work intensity of operators is reduced.