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发明公开
CN110669832A 喹诺酮类抗生素抗性基因aac(6’)-ib-cr的特异性引物和应用 审中-实审

喹诺酮类抗生素抗性基因aac(6’)-ib-cr的特异性引物和应用
摘要：
本发明涉及一种喹诺酮类抗生素抗性基因aac(6’)-ib-cr的特异性引物，所述引物的核苷酸序列为：F1：SEQ ID NO:1；R1：SEQ ID NO:2。本引物具有较高的特异性，检测方法的检测过程无需依靠标准曲线来测定核酸量，而是可以直接读取DNA分子的拷贝数，大大简化操作步骤，该方法结果判读直观准确、精确度高、灵敏度高，重复性好，同时具有高效性，最低可检测21个拷贝数。
摘要（英）：

The invention relates to a specific primer of a quinolone antibiotic resistance gene aac (6')-ib-cr. The nucleotide sequence of the primer is F1: SEQ ID NO:1; R1:SEQ ID NO:2. The primer has the advantages that the specificity is higher; the detection process of a detection method does not need to depend on a standard curve to determine the amount of nucleic acid, but can directly read the copy number of DNA molecules, so that the operation steps are greatly simplified, and the method is intuitive and accurate in result interpretation, high in precision, high in sensitivity, good in repeatability and high in efficiency, and can detect 21 copy numbers at least.

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