The invention discloses a vehicle, a motor controller and an over-current protection circuit. The over-current protection circuit comprises a detection module used for detecting a current signal of protection equipment and converting the current signal into a voltage signal, an amplifier used for amplifying the voltage signal, and a control module used for turning off the protection equipment whenthe voltage value corresponding to the amplified voltage signal is greater than a preset voltage threshold. According to the over-current protection circuit provided by the embodiment of the invention, the protection equipment can be quickly turned off when the over-current point of the protection equipment is detected, so that the over-current protection problem caused by short circuit of a motor phase line or a locked rotor is solved, and the safety of the circuit is greatly improved. Moreover, the over-current protection circuit is simple and easy to implement.