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发明公开
CN110699461A 梅花参的荧光PCR检测方法及其引物和探针 审中-实审

梅花参的荧光PCR检测方法及其引物和探针
摘要：
本发明涉及梅花参的实时荧光PCR检测方法。基于具有高度物种特异性的梅花参线粒体细胞色素氧化酶亚基I序列(GenBank:FJ589205.1)设计了具有物种特异性的引物和探针，实现以Real-Time PCR的方法对梅花参进行物种鉴定。本方法由于其引物和探针具有较高的特异性和灵敏度，可用于鉴别深加工后海参制品的真伪。
摘要（英）：

The invention relates to a real-time fluorescence PCR detection method of Thelenota ananas; and moreover, primers and a probe with species specificity are designed on basis of a highly species-specific Thelenota ananas mitochondrial cytochrome oxidase subunit I sequence (GenBank: FJ589205.1) so as to realize species identification on the Thelenota ananas by adopting a Real-Time PCR method. Owe torelatively high specificity and sensitivity of the primers and the probe, the real-time fluorescence PCR detection method of the Thelenota ananas can be used for identifying authenticity of deep processed sea cucumber products.

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