The invention discloses a tobacco flaky cut stem standard sample card and a manufacturing method thereof. The standard sample card comprises a plurality of flaky cut stem standard samples and a plastic encapsulation body, wherein the plurality of flaky cut stem standard samples are sealed in the plastic encapsulation body; the plastic encapsulation body is made of a transparent material; the plurality of flaky cut stem standard samples are in two groups; the first group comprises at least three flaky cut stem standard samples; the minimum value of the length of the flaky cut stem standard samples in the first group is 7mm, and the maximum value of the length of the flaky cut stem standard samples in the first group is 20mm; and the second group comprises at least three flaky cut stem standard samples, the minimum value of the width of the flaky cut stem standard samples in the second group is 5mm, and the maximum value of the width of the flaky cut stem standard samples in the second group is 8mm. A visual measure is used; a flaky form fast detection and judging method suitable for different cut stems is created, so that the adaptability of the cut stems in a product is higher; quality identification can be fast made; and the stability of the production process can be effectively supervised.