The invention belongs to the technical field of application of a growth regulator in fresh grape cultivation, and discloses a treatment method for expanding and hardening flame seedless grape fruit grains. The treatment method comprises the steps of strengthening field cultivation management and branch and vine management, lengthening inflorescences, carrying out rainproof cultivation in rainy areas, and controlling diseases; scientifically expanding fruits, on the basis of elongation of inflorescences and after physiological fruit falling, spraying 20% gibberellic acid 5000-7500 times liquidfor the first time when the flameless seedless grape fruit grains are slightly larger than soybean grains, and spraying 5000 times liquid once 10-15 days later so that the flameless seedless grape fruit grains are expanded, obviously hardened and resistant to transportation. Meanwhile, the coloring is good, the commodity rate is improved, and therefore, the original flame seedless grapes with highquality, early maturity, no pressure resistance and no transportation resistance have the condition of large-area commercial production.