The invention belongs to the field of livestock breeding and Chinese veterinary medicines, and particularly relates to pet Chinese herbal medicine cat food for adult sterilization male pet cat healthcare, and a preparation method thereof. The Chinese herbal medicine cat food is prepared from a staple food grain composition and a traditional Chinese medicine composition according to the weight ratio being 50:1, wherein the staple food grain composition is prepared from the following raw materials in parts by weight: 20 parts of muscle type animal component (lean meat), 2 to 5 parts of fat-freeheart, 1 to 5 parts of liver, 0.1 to 1 part of bone meal or eggshell powder, and 1 to 5 parts of collagen-rich tissues of tendon or fur; and the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 1 part of ginseng, 1 part of roasted rhizoma atractylodis macrocephalae, 1 part of Chinese yam, 2 parts of coreless semen nelumbinis, 2 parts of white hyacinth bean, 1 part of radix glycyrrhizae preparata, and 2 parts of semen sesami nigrum. The Chinese herbal medicine cat food adopts traditional Chinese medicines with homology of medicine and food matched with grain free cat food for conditioning so as to be capable of tonifying spleen, resolving dampness, nourishing kidney and nourishing blood, and have the efficacies on preventing osteoporosis, improving a digestive system function, brightening fur and feather, improving metabolism, resisting oxidation and the like, so that the living quality of adult male pet cats after sterilizationis improved.