The invention provides a preparation method of a bifida ferment filtrate composition, and relates to the technical field of microbial fermentation and cosmetics. The preparation method of the bifida ferment filtrate composition comprises the steps of (1) inoculating a bifidobacterium activated by a shaking table into a culture medium, and fermenting; (2) after finishing fermenting, inactivating abacteria solution, and collecting a liquid supernatant after low-speed centrifugation; (3) extracting essences from tremella fuciformis at high temperature, stirring and filtering, and adding a preservative; (4) smashing wild soybean, carrying out ultrasonic extraction, heating extraction and backflow stirring extraction, filtering, concentrating, separating, purifying, and preparing a butanediolsolution; and (5) taking fermentation liquor in the step (2), adding hyaluronic acid, nicotinamide, the tremella fuciformis extract in the step (3) and the wild soybean extract in the step (4) with different molecular weights according to the proportion, heating, stirring and dissolving to obtain the final composition. The preparation method of the bifida ferment filtrate composition provided by the invention can effectively solve the problems of high chemical pollution and insufficient tenderness and activity existed in cosmetics.