The invention discloses a preparation method of a Yao medicine extract for nourishing blood and blacking hair, and belongs to the technical field of traditional Chinese medicine.The preparation methodof the Yao medicine extract comprises the following steps: step 1: weighing the following raw materials, crushing the raw materials respectively, and mixing the crushed raw materials to obtain a mixed medicinal material; step 2: adding the mixed medicinal material to water, conducting soaking, then adding water, performing extracting 1-3 times, 4 hours each time, and combining the extracts; and step 3: concentrating the extract under reduced pressure to obtain an extractum, conducting canning and sterilizing, and obtaining the Yao medicine extract for nourishing blood and blacking hair. According to the present invention, the Yao medicine extract for nourishing blood and blacking hair is formulated from a variety of traditional Chinese medicines, the composition is reasonable, the compatibility is proper, the effective ingredients of each component can be fully released, and the Yao medicine extract has the effects of nourishing blood, blacking hair, detoxifying and calming nerves, and can be used for activating blood circulation, generating new hair, and turning white hair to black hair.