The invention discloses an artificial propagation method of Glyptothorax sinensis. The method includes the steps of firstly, collecting wild parent Glyptothorax sinensis; secondly, domesticating the wild parent Glyptothorax sinensis; thirdly, cultivating the parent Glyptothorax sinensis; fourthly, performing artificial spawning induction; fifthly, performing artificial incubation. The method has the advantages the parent Glyptothorax sinensis is cultivated by the artificial propagation method, a large number of tests are performed in each of the steps of parent Glyptothorax sinensis collecting, domestication and cultivation to obtain the optimal propagation method, parent Glyptothorax sinensis domestication survival rate reaches 70-80%, and technical support is provided for large-scale artificial Glyptothorax sinensis propagation; in addition, after the artificial medicine injection spawning induction, the parent Glyptothorax sinensis is placed in an artificially created wild-simulatedenvironment, a good environment is provided for the natural spawning and fertilization of the parent Glyptothorax sinensis, fish egg fertilization rate is guaranteed, artificial propagation failure caused by male Glyptothorax sinensis aspermatism, the spawning induction rate of the method is 70-75%, the fertilization rate of the method is 70-90%, and the incubation rate of the method is 70-80%.