The invention discloses a barrier strip for highway reconstruction engineering construction in uninterrupted traffic. The barrier strip for the highway reconstruction engineering construction in the uninterrupted traffic comprises four I-shaped steels, wherein the two I-shaped steels on each side are set as a group, two transverse plates are detachably connected between opposite side walls of thetwo I-shaped steels in each group, one of the transverse plates in each group is located at the upper end of space between the two I-shaped steels in each group, and the other transverse plate in eachgroup is located at the lower end of the space between the two I-shaped steels in each group. The invention further provides a use method of the barrier strip for the highway reconstruction engineering construction in the uninterrupted traffic. According to barrier strip for the highway reconstruction engineering construction in the uninterrupted traffic and the use method thereof, the purpose ofrapidly installing the barrier strip is achieved, installing speed is greatly improved, the quantity of the barrier strips can be confirmed according to the length of a construction road segment, andmeanwhile buttering and protection functions are further realized for vehicles which are under accident circumstances, and therefore collision force of the vehicles is reduced, the security coefficient of the vehicles is improved, stability of the overall structure is improved, and the circumstance that the barrier strip topples over and influences construction effects is prevented from occurring.