The invention discloses a connection structure of a printing board and a die-cutting device of the connection structure. The connection structure of the printing board includes the printing board body, the printing surface of the printing board body is provided with a plurality of cutting openings and a plurality of gaps, the two adjacent cutting openings communicate by the gaps, the multiple cutting openings and the multiple gaps are arranged in a row at intervals to form a connection line, and the product appearance is protected. According to the connection structure, the connection part ofthe printing board avoids the printing surface, the phenomena such as folding fault, color explosion and off ink of the printing surface are avoided. The die-cutting device of the connection structureof the printing board includes a die-cutting cutter and a steel bottom die, the die-cutting cutter is located above the steel bottom die, the die-cutting cutter is fixed to a moving substrate of a die-cutting machine, the steel bottom die is arranged on a driving device reciprocating up and down of the die-cutting machine, and fast preparation of the connecting line which can avoid the printing surface is realized. According to a preparation method of the connection structure of the printing board, through die-cutting, the connection structure is efficiently prepared and connected.