The embodiment of the invention discloses an ecological flow guarantee method and device, electronic equipment and a computer storage medium. The method comprises the steps that whether the ecologicalflow of a target river is smaller than preset ecological flow is judged; if the ecological flow is smaller than the preset ecological flow, obtaining an ecological water replenishing optimal configuration result by utilizing an interval multi-objective planning model, and replenishing water according to the ecological water replenishing optimal configuration result until the ecological flow reaches the preset ecological flow; wherein the interval multi-objective planning model is a model constructed based on an objective function and constraint conditions, the objective function comprises a pollutant discharge amount objective function, and the constraint conditions comprise that the pollutant discharge concentration is not greater than a preset control section target concentration. According to the ecological flow guaranteeing method and device, the electronic equipment and the computer storage medium, the water quality of the ecological flow needed by the river can be guaranteed, and therefore the clean water source of the river is prevented from being polluted, and the ecological environment is prevented from being damaged.