A display substrate having a display area and a peripheral area is provided. The display substrate includes: a plurality of light emitting elements on a base substrate and in the display area; a packaging layer positioned on one side, far away from the base substrate, of the plurality of light-emitting elements; and a first separation column in the peripheral area and on the base substrate, the first separation column forming a first fence substantially surrounding a first region. The first separation column includes a plurality of metal layers and a plurality of insulating layers alternatelystacked on the base substrate. A width of a respective one of the plurality of insulating layers in a cross-pillar direction is greater than a width of a respective one of the plurality of metal layers in the cross-pillar direction, the cross-pillar direction spanning the first fence from the display area toward the first separation pillar.