The invention discloses a method for separating and selecting homogeneous temporary maintainer and homozygous sterile lines by using F2 generation seeds of genic three-line rapes. The method directlyselects hybrid varieties (combinations) with good high and stable yield, strong stress resistance, excellent quality and other excellent comprehensive economic properties on the basis of no breeding materials of genic three-line parents (namely temporary maintainer and homozygous sterile lines) for bagging and selfing. According to the different fertility performance of F2 generation and F3 generation, methods such as same bag hybridization of fertile and sterile plants, selfing of fertile plants, taking plants of corresponding plant row materials after initial flowering as parents respectively to produce seeds in mosquito nets on the basis of research, and then carrying out fertility identification on the generated corresponding seeds and the like are adopted respectively, and the methodof directly and rapidly separating and selecting homogeneous temporary maintainer and homozygous sterile lines within 3-4 generations is adopted.