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发明公开
CN110787318A 一种具有免疫成骨功能的人工韧带及其制备方法 无效 - 驳回

一种具有免疫成骨功能的人工韧带及其制备方法
摘要：
本发明公开了一种具有免疫成骨功能的人工韧带及其制备方法，所述制备方法包括步骤：(1)把PET人工韧带放在超声机中，清洗，晾干；(2)把步骤(1)得到的PET人工韧带进行裁剪，将裁剪好的PET人工韧带放入盛有Tris-HCL溶液的烧杯中；再把25mL多巴胺盐酸盐溶液加入烧杯中，反应时间1-24h，得PDA修饰的PET韧带；(3)把步骤(2)得到的PDA修饰的PET韧带放入50mL离心管中，再加入CS-ADH溶液，得CS-PDA修饰的PET韧带；(4)将1-20ug的rhBMP-2溶液缓慢均匀滴加到步骤(3)得到的PET韧带表面，-20℃冰冻过夜，在无菌冻干机中冷冻干燥，即得。本申请采用多巴胺的自聚合反应吸附在PET上，然后通过多巴胺与硫酸软骨素-ADH的反应将硫酸软骨素连接PET纤维表面，从而赋予PET人工韧带免疫调控功能。
摘要（英）：

The invention discloses an artificial ligament having immunization bone formation function and a preparation method of the artificial ligament. The preparation method comprises the steps of (1) placing a PET artificial ligament in an ultrasonic machine, performing cleaning, and performing drying by airing; (2) cutting out the PET artificial ligament obtained in the step (1), placing the PET artificial ligament which is cut out in a beaker loaded with a Tris-HCL solution, adding 25mL of a dopamine hydrochloride solution to the beaker, and performing a reaction for 1-24h, to obtain a PET ligament modified by PDA; (3) placing the PET ligament modified by the PDA, obtained in the step (2) in a 50mL centrifugal tube, and then adding a CS-ADH solution to obtain a PET ligament modified by CS-PDA;and (4) slowly and uniformly dropwise adding 1-20ug of an rhBMP-2 solution to the surface of the PET ligament obtained in the step (3), performing freezing at minus 20 DEG C, performing staying overnight, and performing freeze drying in a bacteria-free freeze dryer so as to obtain the artificial ligament. The dopamine adopted for the artificial ligament is adsorbed to the PET through an autopolymerization reaction, and then through a reaction of the dopamine and chondroitin sulfate-ADH, chondroitin sulfate is connected to the surface of PET fibers, so that an immunoregulation function is provided for the PET artificial ligament.

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