The invention relates to the technical field of nutrient research of mineral elements in grassland plants, in particular to a method of establishing a model for accumulating mineral elements in grassland plants. The method specifically comprises the following steps of material preparation, potted grassland forage grass plant, mineral element liquid mixed fertilizer spraying, sample collection andpretreatment and model establishment. According to the method, the accumulation process of the mineral elements in the grassland plants is greatly accelerated, and development and re-understanding ofan artificial simulation test of mineral element accumulation in the degradation succession process are facilitated. The establishment of the accumulation model of the mineral elements in the grassland plants is beneficial to manually controlling the variety and the accumulation amount of the mineral elements in the pasture, so that qualitative and quantitative research on the relationship betweennutrient supply and the accumulation amount of a certain mineral element in the grassland plants is facilitated; and through preparation of a mineral element spraying fertilizer, the synergistic andantagonistic effects among mineral elements can be artificially reduced, and the influence of a certain mineral element and the supply change of the mineral element on the accumulation amount of the mineral element is further explained through tests.