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发明公开
CN110806421A 一种PAMAM与客体小分子相互作用方式的NMR检测方法 有权

一种PAMAM与客体小分子相互作用方式的NMR检测方法
摘要：
本发明涉及一种PAMAM与客体小分子相互作用方式的NMR检测方法，所述的NMR检测方法是基于一维氢谱、DOSY谱、NOESY谱、CSSFs-TOCSY谱和pure shift谱进行PAMAM与客体小分子相互作用方式的检测，至少选择PAMAM与客体小分子的一维氢谱、DOSY谱和NOESY谱，当所述PAMAM与客体小分子的一维氢谱中出现谱峰重叠难以进行指认与归属时，再进一步选择CSSFs-TOCSY谱和pure shift谱中的一种或两种进行分离重叠谱峰，通过对比PAMAM与客体小分子相互作用前后谱图的变化，即可得知PAMAM与客体小分子的相互作用方式。本发明的检测方法具有所需测试样品量小、测试精确度高、分辨率高，可实现对样品的无损检测等优点。本发明的检测方法针对研究PAMAM与客体小分子相互作用方式中存在的谱峰重叠归属不明确现象提供有效可靠的解决方法。
摘要（英）：

The invention relates to an NMR detection method for an interaction way between PAMAM and object micromolecules. The NMR detection method performs detection on the interaction way between the PAMAM and the object micromolecules on the basis of a one-dimensional hydrogen spectrum, a DOSY spectrum, an NOESY spectrum, a CSSFs-TOCSY spectrum and a pure shift spectrum, the one-dimensional hydrogen spectra, the DOSY spectra and the NOESY spectra of the PAMAM and the object micromolecules are selected at least, when overlapped peaks appear in the one-dimensional hydrogen spectra of the PAMAM and theobject micromolecules and the one-dimensional hydrogen spectra are difficult to identify and attribute, one or two of the CSSFs-TOCSY spectra and the pure shift spectra are further selected for separating the overlapped peaks, and by comparing changes of spectrograms before and after interaction between the PAMAM and the object micromolecules, the interaction way between the PAMAM and the object micromolecules can be obtained. The detection method provided by the invention has the advantages of few required test samples, high test accuracy, high resolution and nondestructive detection on the samples. The detection method provided by the invention provides effective and reliable solution specific to the phenomenon that attribution of the overlapped peaks is undefined during study on the interaction way between the PAMAM and the object micromolecules.

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