The invention relates to an analyte detection method. The present invention provides a technique capable of detecting an analyte without performing B/F separation, and preferably provides a techniquecapable of detecting an analyte in a state in which a non-specific signal is more easily and further reduced without performing B/F separation. Disclosed is an analyte detection method, including detecting an analyte in such a state that a complex composed of the analyte in a sample of interest, a particle capable of binding to the analyte and a trapping substance capable of binding to the analyteis formed on a substrate, wherein the analyte is detected on the basis of an index associated with a behavior of the particle.