The invention relates to a 3D display substrate which comprises a plurality of sub pixels, a driving unit and a plurality of planoconvex lenses positioned at the light emitting sides of the pluralityof sub pixels, wherein the plurality of sub pixels are arranged in a one-to-one correspondence way to the plurality of planoconvex lenses; the light emitting surface of each sub pixel is positioned onthe focal plane of the corresponding planoconvex lens; each sub pixel comprises a plurality of son pixels arranged in an array manner; and the driving unit is used for driving the plurality of son pixels to display images at different gray scales so as to form a 3D display image. The invention also relates to a 3D display device and a display method.