The invention relates to the technical field of skin-care products, in particular to polypeptide lyophilized powder and application thereof in preparing anti-aging cosmetics. The polypeptide lyophilized powder provided herein comprises glycine, trehalose, mannitol, serum albumin, sodium dihydrogen phosphate, disodium hydrogen phosphate, polysorbate-20 and a polypeptide, wherein the polypeptide isselected from tripeptide-1, tripeptide-2, hexapeptide-9, hexapeptide-11, acetyl pentapeptide-1, glycyl-l-histidyl-l-lysine and carnosine. The polypeptide lyophilized powder according to the inventionhas good solubility, can increase the yield of collagen, can improve facial fine lines and brighten the skin, and therefore, has an anti-aging function.