The invention relates to the technical field of biology, in particular to a method for preparing uridine diphosphate glucose by means of an immobilized enzyme method. The method specifically comprisesthe steps of: (1) preparing UDPG (uridine diphosphate glucose) production enzymes; (2) immobilizing the UDPG production enzymes; and (3) separating a product. According to the invention, two UDPG production enzymes UGP and SPA are adopted, and the UDPG can be synthesized by just two-step enzymatic reaction; compared with a complex process for producing UDPG by chemical and fermentation methods, the method here has a simpler reaction process, with the reaction easier to control, and the product quality being more stable; the UDPG is prepared by means of an immobilized enzyme catalysis method,and the immobilized enzymes can be continuously and repeatedly used many times, so that the production cost is greatly reduced; in addition, a large amount of pigments, other types of nucleotides andother impurities introduced by the use of yeast are avoided, so that purification is easier; the method is suitable for large-scale production of UDPG.