The invention discloses heat-resistant alloy single wires and a preparation method thereof. The alloy single wires comprise the following components of, in percentage by mass, 0.01-0.05 wt% of Cu, 0.01-0.05 wt% of Sc, 0.01-0.03 wt% of B, and the balance Al and inevitable impurities. According to the heat-resistant alloy single wires and a preparation method thereof, the industrial aluminum ingot with the purity of more than or equal to 99.7% is used as a raw material, and the microstructure and the comprehensive performance of the alloy are improved by adding traces Sc elements, and the treatment process is optimized, so that the IACS (20 DEG C) with the electrical conductivity of more than or equal to 61% is developed, the tensile strength is more than or equal to 160 MPa, the elongationis more than or equal to 2.0%, the long-term heat-resistant temperature is more than or equal to 120 DEG C, the temperature is maintained at 210 DEG C for 1 h, and the strength residual rate of the heat-resistant alloy single wires is more than or equal to 90%.